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Because plants absorb carbon from the atmosphere during photosynthesis, and as animals eat plants, the animals will also contain the same level of C in a sample with that in "modern" material, defined as 1950 AD.We can equally well use a different standard if we know its relation to "modern," or 1950 AD.Subsequent developments made this method obsolete, and more accurate methods using gas-proportional counters and liquid-scintillation counters were developed.These methods relied on the observation of a decay of the radioactive carbon atoms.The radiocarbon time-scale has now been calibrated with tree rings to more than 10000 years before present, and beyond that using a coral chronology (Stuiver, et al., 1993).The practical use of accelerator mass spectrometry was shown in 1977 by two groups simultaneously at Mc Masversity and at the universities of Toronto and Rochester (N. The great advantage of using AMS is that we can measure the isotope ratio of C to stable carbon directly.Radiocarbon dating: radioactive carbon decays to nitrogen with a half-life of 5730 years.In dead material, the decayed 14C is not replaced and its concentration in the object decreases slowly.

Accelerator mass spectrometry (AMS) is a technique for direct measurement of the concentration of radioisotopes.Since nearly all applications where the precise age is needed require calibration, this difference is removed in the calibration process].Carbon-14 is produced in the upper atmosphere by nuclear reactions induced by cosmic rays on nitrogen (see Fig. Nearly all the carbon in the atmosphere is present as carbon dioxide (CO in the atmosphere maintains an equilibrium with the biosphere and the oceans.This carbon-14 is radioactive and decays with a half-life of 5730 years.For historical reasons, uncalibrated radiocarbon measurements are often referred to a half-life of 5568 years.

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A radiocarbon measurement can be obtained on a sample of ~0.5 mg of carbon, and measured to 40 years in uncalibrated radiocarbon age in a measurement time of 30–40 minutes on each sample.

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